Overview

  • Product nameAnti-RAD52 antibody
    See all RAD52 primary antibodies
  • Description
    Rabbit polyclonal to RAD52
  • Specificityab61211 detects endogenous levels of total RAD52 protein.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non-phosphopeptide derived from human RAD52 around the phosphorylation site of tyrosine 104.

  • Positive control
    • Human testis tissue and 293 cell extracts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab61211 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab61211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

Target

  • FunctionInvolved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
  • Sequence similaritiesBelongs to the RAD52 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA repair protein RAD52 antibody
    • DNA repair protein RAD52 antibody
    • DNA repair protein RAD52 homolog antibody
    • RAD 52 antibody
    • Rad52 antibody
    • RAD52 homolog (S. cerevisiae) antibody
    • RAD52 homolog (S. cerevisiae) antibody
    • RAD52 homolog antibody
    • RAD52_HUMAN antibody
    • Recombination protein RAD52 antibody
    • Recombination protein RAD52 antibody
    • Rhabdomyosarcoma antigen MU RMS 40.23 antibody
    see all

Anti-RAD52 antibody images

  • ab61211 at 1/50 dilution staining RAD52 in human testis by Immunohistochemistry, Paraffin-embedded tissue, in the absence (left) or presence (right) of the immunising peptide.
  • All lanes : Anti-RAD52 antibody (ab61211) at 1/500 dilution

    Lane 1 : 293 cell extracts
    Lane 2 : 293 cell extracts with immunising peptide


    Predicted band size : 46 kDa
    Observed band size : 46 kDa

References for Anti-RAD52 antibody (ab61211)

ab61211 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

Submitted Mar 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"