Recombinant Anti-RAD52 antibody [EPR3464(2)] (ab124971)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3464(2)] to RAD52
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RAD52 antibody [EPR3464(2)]
See all RAD52 primary antibodies -
Description
Rabbit monoclonal [EPR3464(2)] to RAD52 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human RAD52 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa, HEK-293T and PC3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 3.00 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3464(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa). |
Target
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Function
Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase. -
Sequence similarities
Belongs to the RAD52 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5893 Human
- Omim: 600392 Human
- SwissProt: P43351 Human
- Unigene: 410355 Human
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Alternative names
- DNA repair protein RAD52 antibody
- DNA repair protein RAD52 antibody
- DNA repair protein RAD52 homolog antibody
see all
Images
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Lane 1 : Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution
Lane 2 : Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted? -
All lanes : Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 46 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab124971 has been referenced in 8 publications.
- Lippert TP et al. Oncogenic herpesvirus KSHV triggers hallmarks of alternative lengthening of telomeres. Nat Commun 12:512 (2021). PubMed: 33479235
- Zhang JM et al. Alternative lengthening of telomeres is a self-perpetuating process in ALT-associated PML bodies. Mol Cell 81:1027-1042.e4 (2021). PubMed: 33453166
- Liu X et al. Chromosomal aberration arises during somatic reprogramming to pluripotent stem cells. Cell Div 15:12 (2020). PubMed: 33292330
- Hanamshet K & Mazin AV The function of RAD52 N-terminal domain is essential for viability of BRCA-deficient cells. Nucleic Acids Res 48:12778-12791 (2020). PubMed: 33275133
- Gong H et al. p73 coordinates with ?133p53 to promote DNA double-strand break repair. Cell Death Differ N/A:N/A (2018). PubMed: 29511339
- Tong L et al. RDM1 plays an oncogenic role in human lung adenocarcinoma cells. Sci Rep 8:11525 (2018). PubMed: 30069034
- Yasuhara T et al. Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair. Cell 175:558-570.e11 (2018). PubMed: 30245011
- Gong L et al. p53 isoform ?133p53 promotes efficiency of induced pluripotent stem cells and ensures genomic integrity during reprogramming. Sci Rep 6:37281 (2016). PubMed: 27874035