Overview

  • Product name
    Anti-RAD52 antibody [EPR3464(2)]
    See all RAD52 primary antibodies
  • Description
    Rabbit monoclonal [EPR3464(2)] to RAD52
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C-terminus of Human RAD52.

  • Positive control
    • HeLa, 293T, and PC3 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
    • Sequence similarities
      Belongs to the RAD52 family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNA repair protein RAD52 antibody
      • DNA repair protein RAD52 antibody
      • DNA repair protein RAD52 homolog antibody
      • RAD 52 antibody
      • Rad52 antibody
      • RAD52 homolog (S. cerevisiae) antibody
      • RAD52 homolog (S. cerevisiae) antibody
      • RAD52 homolog antibody
      • RAD52_HUMAN antibody
      • Recombination protein RAD52 antibody
      • Recombination protein RAD52 antibody
      • Rhabdomyosarcoma antigen MU RMS 40.23 antibody
      see all

    Images

    • All lanes : Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : PC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 46 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Gong L  et al. p53 isoform ?133p53 promotes efficiency of induced pluripotent stem cells and ensures genomic integrity during reprogramming. Sci Rep 6:37281 (2016). Read more (PubMed: 27874035) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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