Anti-RAD52 (phospho Y104) antibody (ab62458)

Overview

  • Product nameAnti-RAD52 (phospho Y104) antibody
    See all RAD52 primary antibodies
  • Description
    Rabbit polyclonal to RAD52 (phospho Y104)
  • Specificityab62458 detects endogenous levels of RAD52 only when phosphorylated at tyrosine 104.
  • Tested applicationsSuitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human RAD52 around the phosphorylation site of tyrosine 104 (K-F-YP-V-G).

  • Positive control
    • Human testis tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab62458 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab62458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P 1/50 - 1/100.

Target

  • FunctionInvolved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
  • Sequence similaritiesBelongs to the RAD52 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA repair protein RAD52 antibody
    • DNA repair protein RAD52 antibody
    • DNA repair protein RAD52 homolog antibody
    • RAD 52 antibody
    • Rad52 antibody
    • RAD52 homolog (S. cerevisiae) antibody
    • RAD52 homolog (S. cerevisiae) antibody
    • RAD52 homolog antibody
    • RAD52_HUMAN antibody
    • Recombination protein RAD52 antibody
    • Recombination protein RAD52 antibody
    • Rhabdomyosarcoma antigen MU RMS 40.23 antibody
    see all

Anti-RAD52 (phospho Y104) antibody images

  • ab62458, at 1/50 dilution, staining RAD52 in paraffin embedded human testis tissue by Immunohistochemistry in the absence or presence of the immunising peptide.

References for Anti-RAD52 (phospho Y104) antibody (ab62458)

ab62458 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"