Overview

  • Product name
    Anti-Rad53 antibody [EL7.E1]
    See all Rad53 primary antibodies
  • Description
    Mouse monoclonal [EL7.E1] to Rad53
  • Host species
    Mouse
  • Specificity
    This antibody recognises a range of Rad53 isoforms in yeast extracts but does not react with human or mouse Chk2. Other species not tested.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
    Does not react with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Rad53. Yeast RAD53 produced as a recombinant protein in E. coli.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab166859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).

Target

  • Function
    Controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints. Phosphorylates proteins on serine, threonine, and tyrosine. Prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. Seems to be involved in the phosphorylation of RPH1.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.
    Contains 2 FHA domains.
    Contains 1 protein kinase domain.
  • Domain
    FHA domains are phosphothreonine recognition modules, FHA 1 strongly selects for Asp at position +3 relative to phosphothreonine, whereas FHA 2 selects for Ile in this position.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • CHEK2 homolog antibody
      • CHK2 homolog antibody
      • MEC2 antibody
      • RAD53 antibody
      • RAD53_YEAST antibody
      • Serine protein kinase 1 antibody
      • Serine-protein kinase 1 antibody
      • Serine/threonine-protein kinase RAD53 antibody
      • SPK1 antibody
      see all

    Images

    • All lanes : Anti-Rad53 antibody [EL7.E1] (ab166859) at 1 µg/ml

      Lane 1 : TCA preps from S. cerevisiae cells
      Lane 2 : TCA preps from S. cerevisiae cells treated with Hydroxyurea (HU)

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 92 kDa
      Observed band size: 92 kDa
      Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes


      Western Blot showing ab166859 detecting both unphosphorylated and phosphorylated Rad53.

    References

    This product has been referenced in:
    • Schmidt TT  et al. Alterations in cellular metabolism triggered by URA7 or GLN3 inactivation cause imbalanced dNTP pools and increased mutagenesis. Proc Natl Acad Sci U S A 114:E4442-E4451 (2017). WB ; Saccharomyces cerevisiae . Read more (PubMed: 28416670) »
    • Mehta A  et al. Homology Requirements and Competition between Gene Conversion and Break-Induced Replication during Double-Strand Break Repair. Mol Cell 65:515-526.e3 (2017). Read more (PubMed: 28065599) »

    See all 15 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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