Overview

  • Product nameAnti-RAD54 antibody [Rad54 4E3/1]
    See all RAD54 primary antibodies
  • Description
    Mouse monoclonal [Rad54 4E3/1] to RAD54
  • Tested applicationsSuitable for: IP, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberRad54 4E3/1
  • MyelomaSp2/0-Ag14
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 84.3 kDa).
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInvolved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells.
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA repair and recombination protein RAD54 like antibody
    • DNA repair and recombination protein RAD54-like antibody
    • hHR 54 antibody
    • hHR54 antibody
    • HR 54 antibody
    • HR54 antibody
    • hRAD 54 antibody
    • hRAD54 antibody
    • RAD 54A antibody
    • RAD 54L antibody
    • RAD54 (S. cerevisiae) like antibody
    • RAD54 homolog antibody
    • RAD54 like (S. cerevisiae) antibody
    • RAD54 like antibody
    • RAD54 like protein antibody
    • RAD54_HUMAN antibody
    • RAD54A antibody
    • RAD54L antibody
    see all

Anti-RAD54 antibody [Rad54 4E3/1] images

  • Anti-RAD54 antibody [Rad54 4E3/1] (ab11055) at 1/1000 dilution (in blocker + PBS + 0.1% Tween for 4 hours at 4°C) + HCT116p53wt cells (whole cell lysate) at 30 µg

    Secondary
    An Alexa Fluor®680 goat anti-mouse polyclonal at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 84.3 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 94 kDa. We are unsure as to the identity of these extra bands.

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Overlay histogram showing HeLA cells stained with ab11055 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11055, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-RAD54 antibody [Rad54 4E3/1] (ab11055)

This product has been referenced in:
  • Beck C  et al. PARP3 affects the relative contribution of homologous recombination and nonhomologous end-joining pathways. Nucleic Acids Res 42:5616-32 (2014). ICC/IF ; Human . Read more (PubMed: 24598253) »
  • Mason JM  et al. The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors. Cancer Res 74:3546-55 (2014). Read more (PubMed: 24753542) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 40 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa cells)
Total protein in input 200 µg
Specification HeLa cells
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

Submitted Apr 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116p53wt cells)
Loading amount 30 µg
Specification HCT116p53wt cells
Gel Running Conditions Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa and SK-N-SH)
Specification HeLa and SK-N-SH
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Aug 25 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"