Overview

  • Product nameAnti-RAD54B antibody
    See all RAD54B primary antibodies
  • Description
    Mouse polyclonal to RAD54B
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant tagged fusion protein: YCFEAPTLAT LDPPHTVHSA PKEVAVSKEQ EEKSDSLVKY FSVVWCKPSK KKHKKWEGDA VLIVKGKSFI LKNLEGKDIG RGIGYKFKEL EKIEEGQTLM , corresponding to amino acids 86-186 of Human RAD54B

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 103 kDa).

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

  • Application notesIs unsuitable for ICC/IF.
  • Target

    • RelevanceThe RAD52 epistasis group genes are involved in homologous recombination, and they are conserved from yeast to humans. RAD54B is involved in DNA repair and mitotic recombination.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • DNA repair and recombination protein RAD54B antibody
      • EC 3.6.1.- antibody
      • Fibrinogen silencer binding protein antibody
      • FSBP antibody
      • RAD54 homolog B antibody
      see all

    Anti-RAD54B antibody images

    • All lanes : Anti-RAD54B antibody (ab43702) at 1/1000 dilution

      Lane 1 : a total protein extract from E coli with 50ng to 100ng of a Tagged irrelevant antigen (Negative control)
      Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (Tagged fusion protein)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) HRP at 1/5000 dilution

      Predicted band size : 103 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    References for Anti-RAD54B antibody (ab43702)

    ab43702 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Methanol
    Permeabilization Yes - 0.5% TritonX100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Nov 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"