Overview

  • Product name
  • Description
    Rabbit polyclonal to RAD54B
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 861-910 (NSLKPLSMSQ LKQWKHFSGD HLNLTDPFLE RITENVSFIF QNITTQATGT) of human RAD54B (NP_036547).

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution. Titer in peptide based assay is 1/1562500.
ICC/IF 1/500.

Target

  • Relevance
    The RAD52 epistasis group genes are involved in homologous recombination, and they are conserved from yeast to humans. RAD54B is involved in DNA repair and mitotic recombination.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • DNA repair and recombination protein RAD54B antibody
    • EC 3.6.1.- antibody
    • Fibrinogen silencer binding protein antibody
    • FSBP antibody
    • RAD54 homolog B antibody
    • RDH54 antibody
    see all

Images

  • Anti-RAD54B antibody (ab83446) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 103 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • ab83446 (1/500) staining RAD54B in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

References

ab83446 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton x100
Username

Dr. Kirk Mcmanus

Verified customer

Submitted May 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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