Overview

  • Product nameAnti-RAD54B antibody
    See all RAD54B primary antibodies
  • Description
    Rabbit polyclonal to RAD54B
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 861-910 (NSLKPLSMSQ LKQWKHFSGD HLNLTDPFLE RITENVSFIF QNITTQATGT) of human RAD54B (NP_036547).

  • Positive control
    • HeLa cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution. Titer in peptide based assay is 1/1562500.
ICC/IF 1/500.

Target

  • RelevanceThe RAD52 epistasis group genes are involved in homologous recombination, and they are conserved from yeast to humans. RAD54B is involved in DNA repair and mitotic recombination.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • DNA repair and recombination protein RAD54B antibody
    • EC 3.6.1.- antibody
    • Fibrinogen silencer binding protein antibody
    • FSBP antibody
    • RAD54 homolog B antibody
    see all

Anti-RAD54B antibody images

  • Anti-RAD54B antibody (ab83446) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 103 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • ab83446 (1/500) staining RAD54B in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

References for Anti-RAD54B antibody (ab83446)

ab83446 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton x100
Username

Dr. Kirk McManus

Verified customer

Submitted May 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"