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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab86063 was affinity purified using an epitope specific to RAD54L2 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 163 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Function DNA helicase that modulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner. Not able to remodel mononucleosomes in vitro.
Sequence similarities Belongs to the SNF2/RAD54 helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Domain Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to be important for the association with nuclear receptors.
Post-translational modifications Sumoylated.
Cellular localization Nucleus. Localizes in speckle-like nuclear compartments.
Information by UniProt
Androgen receptor interacting protein 4 antibody
Androgen receptor-interacting protein 4 antibody
Anti-RAD54L2 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAD54L2 antibody (ab86063)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling RAD54L2 with ab86063 at 1/1200 (1µg/ml). Detection: DAB.
Western blot - RAD54L2 antibody (ab86063)
All lanes : Anti-RAD54L2 antibody (ab86063) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Predicted band size : 163 kDa Observed band size : 190 kDa ( why is the actual band size different from the predicted?) Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands. Exposure time : 30 seconds
Immunoprecipitation - RAD54L2 antibody (ab86063)
Detection of RAD54L2 by Western Blot of Immunprecipitate.
ab86063 at 1µg/ml staining RAD54L2 in HeLa whole cell lysate immunoprecipitated using ab86063 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.
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