Overview

  • Product name
  • Description
    Rabbit polyclonal to RAD54L2
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Chinese hamster, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 75 and 125 of human RAD54L2 (NP_055921.2)

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 163 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    DNA helicase that modulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner. Not able to remodel mononucleosomes in vitro.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Domain
    Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to be important for the association with nuclear receptors.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localization
    Nucleus. Localizes in speckle-like nuclear compartments.
  • Information by UniProt
  • Database links
  • Alternative names
    • Androgen receptor interacting protein 4 antibody
    • Androgen receptor-interacting protein 4 antibody
    • Arip4 antibody
    • ARIP4_HUMAN antibody
    • HCMV interacting protein; antibody
    • Helicase ARIP4 antibody
    • HSPC325 antibody
    • RAD54 like 2 (S. cerevisiae) antibody
    • RAD54 like protein 2 antibody
    • RAD54-like protein 2 antibody
    • Rad54l2 antibody
    • SRISNF2L antibody
    • Steroid receptor interacting SNF2 domain protein antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling RAD54L2 with ab86063 at 1/1200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-RAD54L2 antibody (ab86063) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 163 kDa
    Observed band size : 190 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of RAD54L2 by Western Blot of Immunprecipitate.
    ab86063 at 1µg/ml staining RAD54L2 in HeLa whole cell lysate immunoprecipitated using ab86063 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

References

This product has been referenced in:
  • Suzuki J  et al. Xkr8 phospholipid scrambling complex in apoptotic phosphatidylserine exposure. Proc Natl Acad Sci U S A 113:9509-14 (2016). WB . Read more (PubMed: 27503893) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab86063.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up