Anti-Rad6 antibody [2782C5] (ab50889)

Overview

  • Product nameAnti-Rad6 antibody [2782C5]
    See all Rad6 primary antibodies
  • Description
    Mouse monoclonal [2782C5] to Rad6
  • Tested applicationsSuitable for: IHC-M, WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Rad6 (internal sequence).

Properties

Applications

Our Abpromise guarantee covers the use of ab50889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-M
WB
Dot blot
  • Application notesDot: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 17 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
    • Information by UniProt
    • Database links
    • Alternative names
      • BHR6A antibody
      • hHR6A antibody
      • HR6A antibody
      • mHR6A antibody
      • MRXS30 antibody
      • MRXSN antibody
      • RAD6 homolog A antibody
      • RAD6A antibody
      • UBC2 antibody
      • UBCD6 antibody
      • UBE2A antibody
      • Ube2a ubiquitin-conjugating enzyme E2A antibody
      • UBE2A_HUMAN antibody
      • Ubiquitin carrier protein A antibody
      • Ubiquitin carrier protein antibody
      • Ubiquitin conjugating enzyme E2 17 kDa antibody
      • Ubiquitin conjugating enzyme E2 21.5 kDa antibody
      • Ubiquitin conjugating enzyme E2A (RAD6 homolog) antibody
      • Ubiquitin conjugating enzyme E2A antibody
      • Ubiquitin protein ligase A antibody
      • Ubiquitin-conjugating enzyme E2 A antibody
      • Ubiquitin-protein ligase A antibody
      see all

    Anti-Rad6 antibody [2782C5] images

    References for Anti-Rad6 antibody [2782C5] (ab50889)

    ab50889 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Oct 14 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"