Overview

  • Product name
  • Description
    Rabbit polyclonal to Rad6
  • Tested applications
    Suitable for: IHC-P, IHC-Mmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human Rad6 (N terminal). Synthetic peptide from the N-terminal of Human Rad6, Swiss-Prot P49459.

  • Positive control
    • Human stomach adenocarcinoma. Mouse squamous cell carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab84395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. The use of citrate buffer will enhance staining. Likely to work in frozen sections.
IHC-M Use at an assay dependent concentration.

Target

  • Function
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt
  • Database links
  • Alternative names
    • BHR6A antibody
    • hHR6A antibody
    • HR6A antibody
    • mHR6A antibody
    • MRXS30 antibody
    • MRXSN antibody
    • RAD6 homolog A antibody
    • RAD6A antibody
    • UBC2 antibody
    • UBCD6 antibody
    • UBE2A antibody
    • Ube2a ubiquitin-conjugating enzyme E2A antibody
    • UBE2A_HUMAN antibody
    • Ubiquitin carrier protein A antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin conjugating enzyme E2 17 kDa antibody
    • Ubiquitin conjugating enzyme E2 21.5 kDa antibody
    • Ubiquitin conjugating enzyme E2A (RAD6 homolog) antibody
    • Ubiquitin conjugating enzyme E2A antibody
    • Ubiquitin protein ligase A antibody
    • Ubiquitin-conjugating enzyme E2 A antibody
    • Ubiquitin-protein ligase A antibody
    see all

Images

  • Detection of Human Rad6 by Immunohistochemistry.
    Sample: FFPE section of Human stomach adenocarcinoma.
    Antibody: ab84395 used at a dilution of 1/250.
    Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1/100.
  • Detection of Mouse Rad6 by Immunohistochemistry.
    Sample: FFPE section of Mouse squamous cell carcinoma.
    Antibody: ab84395 used at a dilution of 1/250.
    Detection: DAB staining using anti Rabbit IHC antibody at a dilution of 1/100.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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