Overview

  • Product nameAnti-Rad9 antibody
    See all Rad9 primary antibodies
  • Description
    Rabbit polyclonal to Rad9
  • SpecificityAb50311 specifically recognizes recombinant RAD9.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:

    PQGPSPVLAEDSEGEG

    corresponding to amino acids 376 - 391 of Human Rad9, conjugated to KLH by a N terminal Cysteine residue linker. The immunizing peptide corresponds to RAD9A and is not present in RAD9B.

  • Positive control
    • WB: extracts of 293T cells transfected with RAD9.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesWhole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).

Target

  • FunctionComponent of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
  • Sequence similaritiesBelongs to the rad9 family.
  • Post-translational
    modifications
    Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell cycle checkpoint control protein antibody
    • Cell cycle checkpoint control protein RAD9A antibody
    • DNA repair exonuclease rad9 homolog A antibody
    • hRAD 9 antibody
    • hRAD9 antibody
    • Rad 9 antibody
    • RAD 9A antibody
    • RAD9 (S pombe) homolog antibody
    • RAD9 homolog A antibody
    • RAD9 homolog antibody
    • Rad9a antibody
    • RAD9A_HUMAN antibody
    see all

References for Anti-Rad9 antibody (ab50311)

ab50311 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"