Overview

  • Product nameAnti-Radixin antibody
    See all Radixin primary antibodies
  • Description
    Rabbit polyclonal to Radixin
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Radixin.

  • Positive control
    • This antibody gave a positive signal in the following lysates: Brain (Human) Tissue Lysate - adult normal tissue; HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab91312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 mg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionProbably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
  • Involvement in diseaseDefects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similaritiesContains 1 FERM domain.
  • DomainThe N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN.
  • Post-translational
    modifications
    Phosphorylated by tyrosine-protein kinases.
  • Cellular localizationCell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
  • Information by UniProt
  • Database links
  • Alternative names
    • CB567 antibody
    • CG12537 antibody
    • DFNB24 antibody
    • ESP10 antibody
    • Hh-induced MATH and BTB domain-containing protein antibody
    • HIB antibody
    • Moesin-B antibody
    • Protein roadkill antibody
    • RADI_HUMAN antibody
    • Radixin antibody
    • RDX antibody
    see all

Anti-Radixin antibody images

  • All lanes : Anti-Radixin antibody (ab91312) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ICC/IF image of ab91312 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab91312 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Radixin antibody (ab91312)

ab91312 has not yet been referenced specifically in any publications.

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