Anti-Radixin antibody [AF1H1] (ab85323)

Overview

  • Product name
    Anti-Radixin antibody [AF1H1]
    See all Radixin primary antibodies
  • Description
    Mouse monoclonal [AF1H1] to Radixin
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    His tagged recombinant fragment, corresponding to amino acids 1-310 of Human Radixin, from E.coli.

  • Positive control
    • A431, HeLa, HepG2, K562, 293T, PC12 and NIH3T3 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.03% Sodium Azide
    Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Clonality
    Monoclonal
  • Clone number
    AF1H1
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
ELISA 1/10000.

Target

  • Function
    Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
  • Involvement in disease
    Defects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similarities
    Contains 1 FERM domain.
  • Domain
    The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN.
  • Post-translational
    modifications
    Phosphorylated by tyrosine-protein kinases.
  • Cellular localization
    Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
  • Information by UniProt
  • Database links
  • Alternative names
    • CB567 antibody
    • CG12537 antibody
    • DFNB24 antibody
    • ESP10 antibody
    • Hh-induced MATH and BTB domain-containing protein antibody
    • HIB antibody
    • Moesin-B antibody
    • Protein roadkill antibody
    • RADI_HUMAN antibody
    • Radixin antibody
    • RDX antibody
    see all

Anti-Radixin antibody [AF1H1] images

  • All lanes : Anti-Radixin antibody [AF1H1] (ab85323) at 1/10000 dilution

    Lane 1 : A431 cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : HepG2 cell lysates
    Lane 4 : K562 cell lysates
    Lane 5 : 293T cell lysates
    Lane 6 : PC12 cell lysates
    Lane 7 : NIH3T3 cell lysates


    Predicted band size : 69 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

References for Anti-Radixin antibody [AF1H1] (ab85323)

ab85323 has not yet been referenced specifically in any publications.

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I apologise for the delay, I contacted my lab colleagues to check whether this antibody has ever been tested in IP applications. They confirmed it was once used in the past with bad results, therefore we wouldn&rs...

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Unfortunately, the exact concentration of the Anti-Radixin antibody [AF1H1] cannot be determined.

This monoclonal antibody isproduced by ammonium sulfate precipitation from ascites. Different lots of thi...

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