- Datasheet
- Specific References (1)
- Protocols
Overview
-
Product nameAnti-RAE1 antibody
See all RAE1 primary antibodies -
DescriptionGoat polyclonal to RAE1
-
Host speciesGoat
-
Tested applicationsSuitable for: WB, IHC-Pmore details
-
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Xenopus laevis, Zebrafish
-
Immunogen
Synthetic peptide:
NAAEELKPRNKK
, corresponding to C terminal amino acids 357-368 of Human RAE1 -
Positive control
- HepG2, HeLa, Jurkat, MCF7, Human brain cortex
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab36139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38-40 kDa (predicted molecular weight: 41 kDa). | |
| IHC-P | Use a concentration of 3 - 6 µg/ml. |
Target
-
FunctionBinds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.
-
Sequence similaritiesBelongs to the WD repeat rae1 family.
Contains 4 WD repeats. -
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
-
Database links
- Entrez Gene: 513080 Cow
- Entrez Gene: 485942 Dog
- Entrez Gene: 8480 Human
- Entrez Gene: 66679 Mouse
- Entrez Gene: 100125839 Pig
- Entrez Gene: 362281 Rat
- Entrez Gene: 393973 Zebrafish
- Omim: 603343 Human
see all -
Alternative names
- dJ481F12.3 antibody
- dJ800J21.1 antibody
- FLJ30608 antibody
see all
Images
-
Western blot - Anti-RAE1 antibody (ab36139)All lanes : Anti-RAE1 antibody (ab36139) at 0.1 µg/ml
Lane 1 : HepG2
Lane 2 : HeLa
Lane 3 : Jurkat
Lane 4 : MCF7
Lysates/proteins at 35 µg per lane.
Predicted band size: 41 kDa
Observed band size: 40 kDa (why is the actual band size different from the predicted?)35µg protein in RIPA buffer
Primary incubation was 1 hour
Detected by chemiluminescence
-
Western blot - Anti-RAE1 antibody (ab36139)Anti-RAE1 antibody (ab36139) at 0.03 µg/ml + HepG2 lysate (RIPA buffer) at 30 µg
Predicted band size: 41 kDa
Observed band size: 41 kDaPrimary incubated for 1 hour. Detected using chemiluminescence.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAE1 antibody (ab36139)ab50411 at 3.8 µg/ml staining RAE1 in Human brain cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.
References
This product has been referenced in:
- Cuende J et al. Retinoic acid downregulates Rae1 leading to APC(Cdh1) activation and neuroblastoma SH-SY5Y differentiation. Oncogene 27:3339-44 (2008). WB . Read more (PubMed: 18212744) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"