Anti-Raf1 antibody (ab230850)
Key features and details
- Rabbit polyclonal to Raf1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Raf1 antibody
See all Raf1 primary antibodies -
Description
Rabbit polyclonal to Raf1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Orangutan -
Immunogen
Synthetic peptide corresponding to Mouse Raf1 aa 50-150 (N terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q99N57 -
Positive control
- WB: Neuro-2a and Jurkat whole cell lysate (ab7899). IHC-P: Human skin tissue. Flow Cyt (Intra): Neuro-2a cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/50.
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WB |
1/1000. Predicted molecular weight: 73 kDa.
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IHC-P |
1/100.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/50. |
WB
1/1000. Predicted molecular weight: 73 kDa. |
IHC-P
1/100. |
Target
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Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3. -
Tissue specificity
In skeletal muscle, isoform 1 is more abundant than isoform 2. -
Involvement in disease
Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity. -
Cellular localization
Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. - Information by UniProt
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Database links
- Entrez Gene: 521196 Cow
- Entrez Gene: 5894 Human
- Entrez Gene: 110157 Mouse
- Entrez Gene: 100173732 Orangutan
- Entrez Gene: 24703 Rat
- Omim: 164760 Human
- SwissProt: A7E3S4 Cow
- SwissProt: P04049 Human
see all -
Alternative names
- c Raf antibody
- C-raf antibody
- C-Raf proto-oncogene, serine/threonine kinase antibody
see all
Images
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Anti-Raf1 antibody (ab230850) at 1/1000 dilution + Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 73 kDa -
Formalin-fixed, paraffin-embedded human skin tissue stained for Raf1 using ab230850 at 1/100 dilution in immunohistochemical analysis.
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Anti-Raf1 antibody (ab230850) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 73 kDa -
Intracellular flow cytometric analysis ofNeuro-2a (mouse neuroblastoma cell line) cell line (right histogram) labeling Raf1 with ab230850 at 1/10 dilution compared with a negative control cell line (left histogram). FITC-conjugated donkey anti-rabbit secondary antibodies were used for the analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab230850 has been referenced in 2 publications.
- Zhang Y et al. Transcription factor PAX4 facilitates gastric cancer progression through interacting with miR-27b-3p/Grb2 axis. Aging (Albany NY) 13:16786-16803 (2021). PubMed: 34162761
- Wang S et al. Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells. BMC Cancer 19:1160 (2019). PubMed: 31783811