Overview

  • Product nameAnti-Raf1 antibody [M208]
    See all Raf1 primary antibodies
  • Description
    Mouse monoclonal [M208] to Raf1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to amino acids in the N terminal region of human Raf1.

  • Positive control
    • A431 and Jurkat cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab76201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
ELISA 1/2000.

Target

  • FunctionInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
  • Tissue specificityIn skeletal muscle, isoform 1 is more abundant than isoform 2.
  • Involvement in diseaseDefects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
    Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 RBD (Ras-binding) domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity.
  • Cellular localizationCytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • c Raf antibody
    • C-raf antibody
    • C-Raf proto-oncogene, serine/threonine kinase antibody
    • CMD1NN antibody
    • Craf 1 transforming gene antibody
    • cRaf antibody
    • Craf1 transforming gene antibody
    • EC 2.7.11.1 antibody
    • kinase Raf1 antibody
    • Murine sarcoma 3611 oncogene 1 antibody
    • NS5 antibody
    • Oncogene MIL antibody
    • Oncogene RAF1 antibody
    • OTTHUMP00000160218 antibody
    • OTTHUMP00000207813 antibody
    • OTTHUMP00000209389 antibody
    • Protein kinase raf 1 antibody
    • Proto-oncogene c-RAF antibody
    • Raf 1 antibody
    • Raf 1 proto oncogene serine/threonine kinase antibody
    • RAF antibody
    • Raf proto oncogene serine/threonine protein kinase antibody
    • RAF proto-oncogene serine/threonine-protein kinase antibody
    • RAF-1 antibody
    • RAF1 antibody
    • RAF1_HUMAN antibody
    • Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody
    • TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody
    • v raf 1 murine leukemia viral oncogene homolog 1 antibody
    • v-raf murine sarcoma viral oncogene homolog 1 antibody
    • vraf1 murine leukemia viral oncogene homolog 1 antibody
    see all

Anti-Raf1 antibody [M208] images

  • All lanes : Anti-Raf1 antibody [M208] (ab76201) at 1/500 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : Jurkat cell lysate


    Predicted band size : 73 kDa
    Observed band size : 73 kDa

References for Anti-Raf1 antibody [M208] (ab76201)

This product has been referenced in:
  • De La Garza EM  et al. Raf-1, a potential therapeutic target, mediates early steps in endometriosis lesion development by endometrial epithelial and stromal cells. Endocrinology 153:3911-21 (2012). WB . Read more (PubMed: 22619359) »
  • Braconi C  et al. Hepatitis C virus proteins modulate microRNA expression and chemosensitivity in malignant hepatocytes. Clin Cancer Res 16:957-66 (2010). WB ; Human . Read more (PubMed: 20103677) »

See all 2 Publications for this product

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