Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)


  • Product name
    Anti-Raf1 (phospho S259) antibody [EPR3433(2)]
    See all Raf1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3433(2)] to Raf1 (phospho S259)
  • Host species
  • Specificity
    This antibody only detects Raf1 when phosphorylated at Serine 259.
  • Tested applications
    Suitable for: WB, IHC-P, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Raf1 aa 250-350 (phospho S259) (Cysteine residue). The exact sequence is proprietary.
    Database link: P04049

  • Positive control
    • WB: HEK293 whole cell lysate. IHC-P: Human endometrial carcinoma and human skeletal muscle tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents


Associated products


Our Abpromise guarantee covers the use of ab173539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 73 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Dot blot Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
    • Tissue specificity
      In skeletal muscle, isoform 1 is more abundant than isoform 2.
    • Involvement in disease
      Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
      Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
    • Sequence similarities
      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
      Contains 1 RBD (Ras-binding) domain.
    • Post-translational
      Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity.
    • Cellular localization
      Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes.
    • Information by UniProt
    • Database links
    • Alternative names
      • c Raf antibody
      • C-raf antibody
      • C-Raf proto-oncogene, serine/threonine kinase antibody
      • CMD1NN antibody
      • Craf 1 transforming gene antibody
      • cRaf antibody
      • Craf1 transforming gene antibody
      • EC antibody
      • kinase Raf1 antibody
      • Murine sarcoma 3611 oncogene 1 antibody
      • NS5 antibody
      • Oncogene MIL antibody
      • Oncogene RAF1 antibody
      • OTTHUMP00000160218 antibody
      • OTTHUMP00000207813 antibody
      • OTTHUMP00000209389 antibody
      • Protein kinase raf 1 antibody
      • Proto-oncogene c-RAF antibody
      • Raf 1 antibody
      • Raf 1 proto oncogene serine/threonine kinase antibody
      • RAF antibody
      • Raf proto oncogene serine/threonine protein kinase antibody
      • RAF proto-oncogene serine/threonine-protein kinase antibody
      • RAF-1 antibody
      • RAF1 antibody
      • RAF1_HUMAN antibody
      • Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody
      • v raf 1 murine leukemia viral oncogene homolog 1 antibody
      • v-raf murine sarcoma viral oncogene homolog 1 antibody
      • v-raf-1 murine leukemia viral oncogene-like protein 1 antibody
      • vraf1 murine leukemia viral oncogene homolog 1 antibody
      see all


    • All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/5000 dilution

      Lane 1 : HEK293 whole cell lysate
      Lane 2 : HEK293 whole cell lysate treated with 100nM Phorbol-12-myristate-13-acetate for 1 hour
      Lane 3 : HEK293 whole cell lysate treated with 100nM Phorbol-12-myristate-13-acetate for 1 hour then incubated with phosphatase

      Lysates/proteins at 15 µg per lane.

      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 73 kDa
      Observed band size: 73 kDa

      Exposure time: 1 second

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling Raf1 with ab173539 at a 1/50 dilution.

    • Dot blot analysis of Raf1 (pS259) peptide (Lane 1) and Raf1 non-phospho peptide (Lane 2) labelling Raf1 (pS259) with ab173539 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Raf1 with ab173539 at a 1/50 dilution.

    • All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/1000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : 293T cell lysates treated with Okadaic Acid + Calyculin A

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 73 kDa


    This product has been referenced in:
    • Narov K  et al. The dual PI3K/mTOR inhibitor GSK2126458 is effective for treating solid renal tumours inTsc2+/-mice through suppression of cell proliferation and induction of apoptosis. Oncotarget 8:58504-58512 (2017). Mouse . Read more (PubMed: 28938574) »

    See 1 Publication for this product

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