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Our Abpromise guarantee covers the use of ab3611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 1 - 2 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42.6 kDa).Can be blocked with Rat RAGE peptide (ab41778).|
|IHC-P||1/10 - 1/100.|
ab3611 at a 2µg/ml concentration staining ~ 45 kDa RAGE in mouse lung lysate by Western blot (ECL).This antibody detects two bands in the 45 kDa range representing the RAGE protein pre and post-glycosylation in mouse lung extract. This antibody also detects an ~25 kDa protein that is believed to be proteolytic degradation product.
Ab3611 used in IHC (frozen) in transgenic mouse retinas.