Our Abpromise guarantee covers the use of ab37647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
|Flow Cyt||Use 1µg for 106 cells. Use PBS/EDTA to detach cells to preserve the glycoproteins on the cell surface; do not fix, do not permeabilise.
ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ELISA||1/2000 - 1/10000.|
This image is courtesy of an anonymous AbreviewThe band at 52kDa is a tubulin loading control.
ab37647 staining human Jurkat T cells by Flow Cytometry. The cells were prepared in PBS with 0.2% BSA. The primary antibody diluted 1/100 and incubated with sample for 30 minutes at 0°C. The secondary antibody was Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/200.
Specimen tube 006 is negative control