Our Abpromise guarantee covers the use of ab54741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein in Western blot|
|Flow Cyt||1/100. See Abreview.
ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Western blot against tagged recombinant protein immunogen using ab54741 RAGE antibody at 1ug/ml. Predicted band size of immunogen is 68 kDa
ab54741 staining human Jurkat T cells by Flow Cytometry. The cells were prepared in PBS with 0.2% BSA. The primary antibody diluted 1/100 and incubated with sample for 30 minutes at 0°C. The secondary antibody was Alexa Fluor® 488 conjugated goat polyclonal to mouse IgG, diluted 1/200.
Specimen tube 001 is negative control.