• Product nameAnti-RAGE antibody
    See all RAGE primary antibodies
  • Description
    Mouse monoclonal to RAGE
  • Tested applicationsSuitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 23-405 of Human RAGE



Our Abpromise guarantee covers the use of ab54741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein in Western blot
Flow Cyt 1/100. See Abreview.

ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • FunctionMediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
  • Tissue specificityEndothelial cells.
  • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Advanced glycosylation end product-specific receptor antibody
    • Ager antibody
    • MGC2235 antibody
    • RAGE_HUMAN antibody
    • Receptor for advanced glycosylation end products antibody
    see all

Anti-RAGE antibody images

  • Western blot against tagged recombinant protein immunogen using ab54741 RAGE antibody at 1ug/ml. Predicted band size of immunogen is 68 kDa

  • All lanes : Anti-RAGE antibody (ab54741) at 1 µg/ml

    Lane 1 : 293T RAGE transfected cell lysate
    Lane 2 : 293T non-transfected cell lysate
  • ab54741 staining human Jurkat T cells by Flow Cytometry. The cells were prepared in PBS with 0.2% BSA. The primary antibody diluted 1/100 and incubated with sample for 30 minutes at 0°C. The secondary antibody was Alexa Fluor® 488 conjugated goat polyclonal to mouse IgG, diluted 1/200.

    Specimen tube 001 is negative control.

    See Abreview

  • ab54741 (1 µg/ml) staining RAGE in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is membrane staining throughout the alveloli.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-RAGE antibody (ab54741)

This product has been referenced in:
  • Durning SP  et al. The Receptor for Advanced Glycation Endproducts Drives T Cell Survival and Inflammation in Type 1 Diabetes Mellitus. J Immunol 197:3076-3085 (2016). Read more (PubMed: 27655844) »
  • Machahua C  et al. Increased AGE-RAGE ratio in idiopathic pulmonary fibrosis. Respir Res 17:144 (2016). WB ; Human . Read more (PubMed: 27816054) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Bel-7402 cells)
Gel Running Conditions Non-reduced Denaturing (10% SDS-PAGE)
Loading amount 50 µg
Specification Bel-7402 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. guoqing zhu

Verified customer

Submitted Jan 04 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step No blocking step used for 30 minute(s) · Temperature: 25°C
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Pepsin
Sample Rat Tissue sections (bone)
Specification bone
Permeabilization No
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Dec 22 2014

We would typically suggest using 106 cells in 100 µl diluted antibody. However, the information regarding how ab54741 has come from an Abreview which one of our customers submitted. Unfortunately, they did not provide any details of exactly ...

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With the two antibodies you have been looking at I would suggest the following antibodies should serve as suitable isotype controls:

Rabbit polyclonal to RAGE (ab37647), rabbit IgG, isotype control: Rabbit IgG- ChIP Grade (ab37415)
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Thank you for your enquiry regarding ab54741.

As the datasheet indicates, this antibody has been tested in WB, Flow Cyt, IHC-P. It has not been characterized specifically in IHC-Fr but it is very likely that ab54741 works on frozen sections. ...

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Thank you for participating in our recent customer satisfaction survey.

I was in touch with our production lab for ab54741 and they said that the concentration of the antibody is different from batch to batch. Please note that even if you rece...

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Thank you for contacting us. It was nice talking to you.

I have discussed the protocol with lab and they have confirmed that they have used microwave antigen retrieval method for 5 minutes with citrate buffer pH 6.0. They have also recommende...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Ich habe zwei Antikörper gefunden, deren Immunogene eine hohe Homologie mit Kaninchen aufweisen und die schon für WB und IHC-P getestet wurden:

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (smooth muscle cells)
Loading amount 50 µg
Specification smooth muscle cells
Treatment 100 ug/ml glycated LDL
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Dr. Laura Toma

Verified customer

Submitted Feb 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (human RAGE cDNA transfected into HEK293 cells)
Loading amount 1 µg
Specification human RAGE cDNA transfected into HEK293 cells
Gel Running Conditions Reduced Denaturing (10-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 21 2008

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