• Product nameAnti-RAGE antibody
    See all RAGE primary antibodies
  • Description
    Rabbit polyclonal to RAGE
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to a sequence mapping in the middle region of human advanced glycosylation end-product-specific receptor(RAGE), different from the mouse and rat sequence by two amino acids.

  • Positive control
    • Rat lung tissue and lysate



Our Abpromise guarantee covers the use of ab65965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesICC: Use at a concentration of 0.5 - 1 µg/ml.
    IHC-P: Use at a concentration of 0.5 - 1 µg/ml.
    WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
    • Tissue specificityEndothelial cells.
    • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Advanced glycosylation end product-specific receptor antibody
      • Ager antibody
      • MGC2235 antibody
      • RAGE_HUMAN antibody
      • Receptor for advanced glycosylation end products antibody
      see all

    Anti-RAGE antibody images

    • Immunohistochemical analysis of paraffin-embedded Rat lung tissue sections using ab65965 at 1µg/ml
    • Anti-RAGE antibody (ab65965) at 2 µg/ml + Rat lung tissue lysate

      Predicted band size : 48 kDa
      Observed band size : 48 kDa

    References for Anti-RAGE antibody (ab65965)

    This product has been referenced in:
    • Wang H  et al. Expression of the receptor for advanced glycation end-products and frequency of polymorphism in lung cancer. Oncol Lett 10:51-60 (2015). IHC-P ; Human . Read more (PubMed: 26170976) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry.

    I am sorry that the image is not very clear. Please rest assured that the positive staining was in the bronchiole respiratory epithelium. I have attached a simlar image found in the following histology book for yo...

    Read More

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Ich habe zwei Antikörper gefunden, deren Immunogene eine hohe Homologie mit Kaninchen aufweisen und die schon für WB und IHC-P getestet wurden:

    Read More