Overview

  • Product nameAnti-RAGE antibody
    See all RAGE primary antibodies
  • Description
    Rabbit polyclonal to RAGE
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to a sequence mapping in the middle region of human advanced glycosylation end-product-specific receptor(RAGE), different from the mouse and rat sequence by two amino acids.

  • Positive control
    • Rat lung tissue and lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab65965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesICC: Use at a concentration of 0.5 - 1 µg/ml.
    IHC-P: Use at a concentration of 0.5 - 1 µg/ml.
    WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
    • Tissue specificityEndothelial cells.
    • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Advanced glycosylation end product-specific receptor antibody
      • Ager antibody
      • MGC2235 antibody
      • RAGE_HUMAN antibody
      • Receptor for advanced glycosylation end products antibody
      see all

    Anti-RAGE antibody images

    • Immunohistochemical analysis of paraffin-embedded Rat lung tissue sections using ab65965 at 1µg/ml
    • Anti-RAGE antibody (ab65965) at 2 µg/ml + Rat lung tissue lysate

      Predicted band size : 48 kDa
      Observed band size : 48 kDa

    References for Anti-RAGE antibody (ab65965)

    ab65965 has not yet been referenced specifically in any publications.

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    I am sorry that the image is not very clear. Please rest assured that the positive staining was in the bronchiole respiratory epithelium. I have attached a simlar image found in the following histology book for yo...

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    Ich habe zwei Antikörper gefunden, deren Immunogene eine hohe Homologie mit Kaninchen aufweisen und die schon für WB und IHC-P getestet wurden:
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