• Product name
    Anti-RAGE antibody [MM0520-8D11]
    See all RAGE primary antibodies
  • Description
    Mouse monoclonal [MM0520-8D11] to RAGE
  • Host species
  • Tested applications
    Suitable for: Flow Cyt, WB, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human RAGE extracellular domain.



Our Abpromise guarantee covers the use of ab89911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 24567328

ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


WB 1/500 - 1/1000. Predicted molecular weight: 48 kDa.
Neutralising Use at an assay dependent concentration.


  • Function
    Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
  • Tissue specificity
    Endothelial cells.
  • Sequence similarities
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Advanced glycosylation end product-specific receptor antibody
    • Ager antibody
    • MGC2235 antibody
    • RAGE_HUMAN antibody
    • Receptor for advanced glycosylation end products antibody
    see all


  • Flow Cytometry analysis of human mesenchymal stem cells labelling RAGE with ab89911. A Phycoerythrin-conjugated F(ab′)2 goat anti-mouse IgG was used as secondary antibody. Histograms were set according to negative control stainings (#).


This product has been referenced in:
  • Vogel S  et al. Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET. J Biol Chem 289:11068-82 (2014). Flow Cyt ; Human . Read more (PubMed: 24567328) »

See 1 Publication for this product

Customer reviews and Q&As

I am sorry to hear that these antibodies are not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed t...

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Thank you for contacting us. I am sorry that this antibody did not perform as stated on the datasheet.  The above amount will be credited back to your credit card.  Our accounting department can be contacted by email at us.credits@abcam.com or by t...

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Thank you for your phone call. I'd be happy to issue you either a credit or refund. Alternatively, a free of charge replacement with another antibody can be sent. I have checked our catalog for other potential suitable RAGE antibodies to ...

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Thank you for your patience. The lab send the follwoing information and suggestion: (1) The immunogen of this antibody is the extracellular domain (based on the UniProt database entry Q15109: residues 24 to 344). (2) The customer...

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Thank you for contacting us. I have passed on your experimental details to the lab for more input, and will let you know what they suggest. Regarding your questions: 1) Which part of the epitope is this antibody raised against? ...

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Thank you for contacting us. Here is the reply I received from the lab. First to answer your question about the testing protocol used by the lab for neutralization studies: The neutralization function of this antibody was tested ...

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Thanks for your inquiry. Our neutralization assay was done in vitro between RAGE and AGE-BAS. It means the antibody indeed blocks the binding of the receptor from AGE in the in vitro setting. I hope this answers your question. Plea...

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Thank you for your calls yesterday, and for your patience. According to the lab, the antibody was tested in vitro using a functional ELISA as follows:   If uninhibited, biotinylated AGE-BSA can bind to immobilized recombinant human RAGE coated on...

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