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|Sample type||Average %||Range|
|Cell culture supernatant||75.89||67% - 82%|
|Serum||102.9||91% - 110%|
|Plasma||82.69||71% - 92%|
Abcam’s RAGE Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse RAGE in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|160X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent||1 x 15ml|
|Biotinylated anti-mouse RAGE||2 vials|
|RAGE Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse RAGE Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Standard curve of mouse RAGE with background signal subtracted (duplicates; +/- SD).
Mouse RAGE measured in in mouse tissue lysates (in RIPA buffer tested in the range of 0.1ug to 0.1 mg protein, quantity of RAGE shown as per mg of extracted protein ), duplicates +/- SD.
Mouse RAGE measured in biological fluids (dilution range neat - 1/10; duplicates +/- SD).
Representative standard curve using ab100738
ab100738 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"