Overview

  • Product nameRAGE Mouse ELISA Kit
    See all RAGE kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    13.72 pg/ml - 10000 pg/ml
  • Recovery

    87 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75.89 67% - 82%
    Serum 102.9 91% - 110%
    Plasma 82.69 71% - 92%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s RAGE Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse RAGE in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionMediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
  • Tissue specificityEndothelial cells.
  • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • Advanced glycosylation end product specific receptor
    • Advanced glycosylation end product-specific receptor
    • Ager
    • DAMA 358M23.4
    • LE 9211 A antigen
    • MAPK MAK MRK overlapping kinase
    • MGC22357
    • MOK
    • OTTHUMP00000174885
    • RAGE 1
    • RAGE isoform NtRAGE-delta
    • RAGE isoform sRAGE-delta
    • RAGE_HUMAN
    • RAGE1
    • Receptor for advanced glycation end products
    • receptor for advanced glycation end-products variant 20
    • Receptor for advanced glycosylation end products
    • Renal tumor antigen
    • Renal tumor antigen 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

RAGE Mouse ELISA Kit images

  • Standard curve of mouse RAGE with background signal subtracted (duplicates; +/- SD).

  • Mouse RAGE measured in in mouse tissue lysates (in RIPA buffer, shown as quantity per mg of extracted protein ), duplicates +/- SD.

  • Mouse RAGE measured in biological fluids (duplicates +/- SD).

  • Representative standard curve using ab100738

Protocols

References for RAGE Mouse ELISA Kit (ab100738)

ab100738 has not yet been referenced specifically in any publications.

Product Wall

Tha lab sent their data for the two lots that gave you low standard OD compared to the June assay.

October


Standard (pg/ml) OD
10,000 2.832
3,333 1.512
1,111 0.732
370.4 0.351
123.5 0.201
41.15 0....

Read More

Thank you for contacting us.

The recommended dilution for testing normal mouse serum and plasma with this kit is 2-fold.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or in...

Read More

Thank you for contacting us.  This ELISA kit has not been validated for use on tissue samples, but may be suitable for that purpose.  Theoretically, any low-salt lysis buffer that meets the following criteria should be compatible with this kit: ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"