RAGE overexpression 293T lysate (whole cell) (ab94079)

Overview

  • Product nameRAGE overexpression 293T lysate (whole cell)
  • Description
    RAGE overexpression lysate
  • General notesab94079 is a 293T cell transfected lysate in which Human RAGE has been transiently over-expressed using a pCMV RAGE plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab94079 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution.

    RAGE overexpression 293T lysate (whole cell) images

    • ab94079 at 15µg/lane on an SDS-PAGE gel.
    • All lanes : Anti-RAGE antibody (ab54741) at 1/500 dilution

      Lane 1 : RAGE 293T Transfected Lysate - Positive Control (ab94079)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

    References for RAGE overexpression 293T lysate (whole cell) (ab94079)

    ab94079 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"