Overview

  • Product nameAnti-RAGEF2 antibody
    See all RAGEF2 primary antibodies
  • Description
    Rabbit polyclonal to RAGEF2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 625 and 675 of human RAGEF2 (NP_057424.2)

  • Positive control
    • HeLa whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 179 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGuanine nucleotide exchange factor (GEF) for Rap1A, Rap2A and M-Ras GTPases. Does not interact with cAMP.
    • Tissue specificityIsoform 3 has highest expression levels in the brain, heart, liver, lung and placenta and is barely detectable in skeletal muscle, kidney and pancreas.
    • Sequence similaritiesContains 1 cyclic nucleotide-binding domain.
      Contains 1 N-terminal Ras-GEF domain.
      Contains 1 PDZ (DHR) domain.
      Contains 1 Ras-associating domain.
      Contains 1 Ras-GEF domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCytoplasm. Cell membrane. Upon binding to M-Ras, it translocates to the plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp667N084 antibody
      • DKFZp686I15116 antibody
      • KIA001LB antibody
      • PDZ domain containing guanine nucleotide exchange factor (GEF) 2 antibody
      • PDZ domain containing guanine nucleotide exchange factor 2 antibody
      • PDZ domain containing guanine nucleotide exchange factor I antibody
      • PDZ domain-containing guanine nucleotide exchange factor 2 antibody
      • PDZ GEF 2 antibody
      • PDZ GEF2 antibody
      • PDZ-GEF2 antibody
      • PDZGEF 2 antibody
      • PDZGEF2 antibody
      • RA GEF 2 antibody
      • RA GEF2 antibody
      • RA-GEF-2 antibody
      • RAGEF 2 antibody
      • Rap guanine nucleotide exchange factor (GEF) 6 antibody
      • Rap guanine nucleotide exchange factor 2 antibody
      • Rap guanine nucleotide exchange factor 6 antibody
      • RAPGEF6 antibody
      • RPGF6_HUMAN antibody
      see all

    Anti-RAGEF2 antibody images

    • All lanes : Anti-RAGEF2 antibody (ab86225) at 0.04 µg

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 179 kDa
      Observed band size : 220 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes
    • Detection of human RAGEF2 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86225 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

      Detection: Chemiluminescence with an exposure time of 30 seconds.

    References for Anti-RAGEF2 antibody (ab86225)

    ab86225 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab86225.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"