Overview

  • Product name
    Anti-RAGEF2 antibody
  • Description
    Rabbit polyclonal to RAGEF2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 625 and 675 of human RAGEF2 (NP_057424.2)

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 179 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Guanine nucleotide exchange factor (GEF) for Rap1A, Rap2A and M-Ras GTPases. Does not interact with cAMP.
    • Tissue specificity
      Isoform 3 has highest expression levels in the brain, heart, liver, lung and placenta and is barely detectable in skeletal muscle, kidney and pancreas.
    • Sequence similarities
      Contains 1 cyclic nucleotide-binding domain.
      Contains 1 N-terminal Ras-GEF domain.
      Contains 1 PDZ (DHR) domain.
      Contains 1 Ras-associating domain.
      Contains 1 Ras-GEF domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm. Cell membrane. Upon binding to M-Ras, it translocates to the plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp667N084 antibody
      • DKFZp686I15116 antibody
      • KIA001LB antibody
      • PDZ domain containing guanine nucleotide exchange factor (GEF) 2 antibody
      • PDZ domain containing guanine nucleotide exchange factor 2 antibody
      • PDZ domain containing guanine nucleotide exchange factor I antibody
      • PDZ domain-containing guanine nucleotide exchange factor 2 antibody
      • PDZ GEF 2 antibody
      • PDZ GEF2 antibody
      • PDZ-GEF2 antibody
      • PDZGEF 2 antibody
      • PDZGEF2 antibody
      • RA GEF 2 antibody
      • RA GEF2 antibody
      • RA-GEF-2 antibody
      • RAGEF 2 antibody
      • Rap guanine nucleotide exchange factor (GEF) 6 antibody
      • Rap guanine nucleotide exchange factor 2 antibody
      • Rap guanine nucleotide exchange factor 6 antibody
      • RAPGEF6 antibody
      • RPGF6_HUMAN antibody
      see all

    Images

    • All lanes : Anti-RAGEF2 antibody (ab86225) at 0.04 µg

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 179 kDa
      Observed band size : 220 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes
    • Detection of human RAGEF2 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86225 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

      Detection: Chemiluminescence with an exposure time of 30 seconds.

    References

    ab86225 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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