Overview

  • Product nameAnti-RAI1 antibody
    See all RAI1 primary antibodies
  • Description
    Rabbit polyclonal to RAI1
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to N terminal residues of Human RAI1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 20738874
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 203 kDa.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceRAI1 (retinoid-acid induced protein 1) may be involved in neuronal differentiation. RAI1 is highly similar to its mouse counterpart and is expressed at high levels mainly in neuronal tissues. RAI1 has a polymorphic polyglutamine tract in it's N-terminal domain. Expression of the mouse counterpart in neurons is induced by retinoic acid. The RAI1 gene is associated with both the severity of the phenotype and the response to medication in schizophrenic patients. Defects in RAI1 are a cause of Smith-Magenis syndrome (SMS). There are four named isoforms.
  • Cellular localizationCytoplasmic and Nuclear. In neurons it is localized to neurites.
  • Database links
  • Alternative names
    • DKFZP434A139 antibody
    • KIAA1820 antibody
    • MGC12824 antibody
    • retinoic acid induced 1 antibody
    • Retinoid acid induced protein 1 antibody
    • SMCR antibody
    • SMS antibody
    see all

Anti-RAI1 antibody images

  • ab58658 staining RAI1 (green) in murine N2a cells transfected with RAI1-HA wild type and mutant forms, by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200). An AlexaFluor®488-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

References for Anti-RAI1 antibody (ab58658)

This product has been referenced in:
  • Carmona-Mora P  et al. Functional and cellular characterization of human Retinoic Acid Induced 1 (RAI1) mutations associated with Smith-Magenis Syndrome. BMC Mol Biol 11:63 (2010). WB, ICC/IF ; Human . Read more (PubMed: 20738874) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"