The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 31, 55, 110 kDa (predicted molecular weight: 110 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/62500.
Tissue specificityHighly expressed in placenta, muscle, kidney and testis. Moderately expressed in heart, brain, lung, liver and intestine. Isoform 2 is widely expressed and expressed in fetal and adult testes, and spermatozoa.
Sequence similaritiesContains 7 ANK repeats.
Cellular localizationCytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the cortical actin cytoskeleton structures in terminal web and cell-cell adhesion sites and stress fibers.