Overview

  • Product name
  • Description
    Rabbit polyclonal to RAI14
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 647-696 (LSQLYKEAQA ELEDYRKRKS LEDVTAEYIH KAEHEKLMQL TNVSRAKAED) of Human RAI14 (NP_056392).

  • Positive control
    • MCF7 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31, 55, 110 kDa (predicted molecular weight: 110 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/62500.

Target

  • Tissue specificity
    Highly expressed in placenta, muscle, kidney and testis. Moderately expressed in heart, brain, lung, liver and intestine. Isoform 2 is widely expressed and expressed in fetal and adult testes, and spermatozoa.
  • Sequence similarities
    Contains 7 ANK repeats.
  • Cellular localization
    Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the cortical actin cytoskeleton structures in terminal web and cell-cell adhesion sites and stress fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ankycorbin antibody
    • Ankyrin repeat and coiled coil structure containing protein antibody
    • Ankyrin repeat and coiled-coil structure-containing protein antibody
    • DKFZp564G013 antibody
    • KIAA1334 antibody
    • NORPEG antibody
    • Novel retinal pigment epithelial cell protein antibody
    • RAI13 antibody
    • RAI14 antibody
    • RAI14_HUMAN antibody
    • Retinoic acid induced 14 antibody
    • Retinoic acid induced protein 14 antibody
    • Retinoic acid-induced protein 14 antibody
    see all

Images

  • Anti-RAI14 antibody (ab83239) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 110 kDa
    Observed band size : 31,55,110 kDa (why is the actual band size different from the predicted?)

References

ab83239 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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