Recombinant Anti-RAI14 antibody [EPR8518] (ab137118)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8518] to RAI14
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RAI14 antibody [EPR8518]
See all RAI14 primary antibodies -
Description
Rabbit monoclonal [EPR8518] to RAI14 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human RAI14 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- HeLa and 293T cell lysates, Human kidney and Human testis tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8518 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Highly expressed in placenta, muscle, kidney and testis. Moderately expressed in heart, brain, lung, liver and intestine. Isoform 2 is widely expressed and expressed in fetal and adult testes, and spermatozoa. -
Sequence similarities
Contains 7 ANK repeats. -
Cellular localization
Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the cortical actin cytoskeleton structures in terminal web and cell-cell adhesion sites and stress fibers. - Information by UniProt
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Database links
- Entrez Gene: 26064 Human
- Entrez Gene: 75646 Mouse
- Entrez Gene: 294804 Rat
- Omim: 606586 Human
- SwissProt: Q9P0K7 Human
- SwissProt: Q9EP71 Mouse
- SwissProt: Q5U312 Rat
- Unigene: 431400 Human
see all -
Alternative names
- Ankycorbin antibody
- Ankyrin repeat and coiled coil structure containing protein antibody
- Ankyrin repeat and coiled-coil structure-containing protein antibody
see all
Images
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All lanes : Anti-RAI14 antibody [EPR8518] (ab137118) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDa -
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling RAI14 with ab137118 antibody at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human testis tissue labelling RAI14 with ab137118 antibody at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab137118 has been referenced in 2 publications.
- Xu J et al. RAI14 Promotes Melanoma Progression by Regulating the FBXO32/c-MYC Pathway. Int J Mol Sci 23:N/A (2022). PubMed: 36233342
- He XY et al. High Expression of Retinoic Acid Induced 14 (RAI14) in Gastric Cancer and Its Prognostic Value. Med Sci Monit 24:2244-2251 (2018). PubMed: 29654694