The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The specific function of RAI2 (Retinoic acid induced 2) has not yet been determined. However, it is suggested that it may have a role in development since retinoic acid is involved in vertebrate anteroposterior axis formation and cellular differentiation, and has been shown to modulate gene expression controlling early embryonic development.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling RAI2 with ab190229 at 10 µg/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.