Overview

  • Product name
    Anti-RAIDD antibody [EPR1639]
    See all RAIDD primary antibodies
  • Description
    Rabbit monoclonal [EPR1639] to RAIDD
  • Tested applications
    Suitable for: WB, IP, ICC, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in human RAIDD

  • Positive control
    • Raji, HeLa, K562 and MCF7 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab76465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 23 kDa.
IP 1/40.
ICC 1/100 - 1/250.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex.
    • Tissue specificity
      Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.
    • Sequence similarities
      Contains 1 CARD domain.
      Contains 1 death domain.
    • Domain
      Contains a death domain involved in the binding of RIP protein.
      The CARD domain mediates the interaction with caspase-2.
    • Information by UniProt
    • Database links
    • Alternative names
      • CASP2 and RIPK1 domain containing adaptor with death domain antibody
      • Caspase and RIP adapter with death domain antibody
      • Caspase and RIP adaptor with death domain antibody
      • Cradd antibody
      • CRADD_HUMAN antibody
      • Death adaptor molecule RAIDD antibody
      • Death domain containing protein CRADD antibody
      • Death domain-containing protein CRADD antibody
      • MGC9163 antibody
      • RIP associated ICH1/CED3 homologous protein with death domain antibody
      • RIP associated protein with a death domain antibody
      • RIP-associated protein with a death domain antibody
      see all

    Images

    • All lanes : Anti-RAIDD antibody [EPR1639] (ab76465) at 1/5000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 23 kDa
      Observed band size : 23 kDa

    References

    This product has been referenced in:
    • Di Donato N  et al. Mutations in CRADD Result in Reduced Caspase-2-Mediated Neuronal Apoptosis and Cause Megalencephaly with a Rare Lissencephaly Variant. Am J Hum Genet 99:1117-1129 (2016). Read more (PubMed: 27773430) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab76465.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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