Overview

  • Product name
    Anti-RAIDD antibody [EPR1640Y]
    See all RAIDD primary antibodies
  • Description
    Rabbit monoclonal [EPR1640Y] to RAIDD
  • Tested applications
    Suitable for: WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues from the C terminus of human RAIDD.

  • Positive control
    • HeLa cell lysate and Human heart muscle tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab52621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IP 1/30.
ICC 1/100 - 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex.
  • Tissue specificity
    Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.
  • Sequence similarities
    Contains 1 CARD domain.
    Contains 1 death domain.
  • Domain
    Contains a death domain involved in the binding of RIP protein.
    The CARD domain mediates the interaction with caspase-2.
  • Information by UniProt
  • Database links
  • Alternative names
    • CASP2 and RIPK1 domain containing adaptor with death domain antibody
    • Caspase and RIP adapter with death domain antibody
    • Caspase and RIP adaptor with death domain antibody
    • Cradd antibody
    • CRADD_HUMAN antibody
    • Death adaptor molecule RAIDD antibody
    • Death domain containing protein CRADD antibody
    • Death domain-containing protein CRADD antibody
    • MGC9163 antibody
    • RIP associated ICH1/CED3 homologous protein with death domain antibody
    • RIP associated protein with a death domain antibody
    • RIP-associated protein with a death domain antibody
    see all

Images

  • Anti-RAIDD antibody [EPR1640Y] (ab52621) at 1/1000 dilution + HeLa cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
  • ab52621, at a 1/50 dilution, staining Human RAIDD in heart muscle, using Immunohistochemistry, Paraffin embedded tissue.

References

This product has been referenced in:
  • Di Donato N  et al. Mutations in CRADD Result in Reduced Caspase-2-Mediated Neuronal Apoptosis and Cause Megalencephaly with a Rare Lissencephaly Variant. Am J Hum Genet 99:1117-1129 (2016). Read more (PubMed: 27773430) »
  • Shen Q  et al. Regulation of CRADD-caspase 2 cascade by histone deacetylase 1 in gastric cancer. Am J Transl Res 6:538-47 (2014). Read more (PubMed: 25360218) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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