Overview

  • Product nameAnti-RAIDD antibody [EPR1640Y]
    See all RAIDD primary antibodies
  • Description
    Rabbit monoclonal [EPR1640Y] to RAIDD
  • Tested applicationsSuitable for: WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues from the C terminus of human RAIDD.

  • Positive control
    • HeLa cell lysate and Human heart muscle tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab52621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IP 1/30.
ICC 1/100 - 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionApoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex.
  • Tissue specificityConstitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.
  • Sequence similaritiesContains 1 CARD domain.
    Contains 1 death domain.
  • DomainContains a death domain involved in the binding of RIP protein.
    The CARD domain mediates the interaction with caspase-2.
  • Information by UniProt
  • Database links
  • Alternative names
    • CASP2 and RIPK1 domain containing adaptor with death domain antibody
    • Caspase and RIP adapter with death domain antibody
    • Caspase and RIP adaptor with death domain antibody
    • Cradd antibody
    • CRADD_HUMAN antibody
    • Death adaptor molecule RAIDD antibody
    • Death domain containing protein CRADD antibody
    • Death domain-containing protein CRADD antibody
    • MGC9163 antibody
    • RIP associated ICH1/CED3 homologous protein with death domain antibody
    • RIP associated protein with a death domain antibody
    • RIP-associated protein with a death domain antibody
    see all

Anti-RAIDD antibody [EPR1640Y] images

  • Anti-RAIDD antibody [EPR1640Y] (ab52621) at 1/1000 dilution + HeLa cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
  • ab52621, at a 1/50 dilution, staining Human RAIDD in heart muscle, using Immunohistochemistry, Paraffin embedded tissue.

References for Anti-RAIDD antibody [EPR1640Y] (ab52621)

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"