Raji whole cell lysate (ab30124)

Overview

  • Product nameRaji whole cell lysate
    See all Raji lysates
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.

  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferpH: 7.90
    Constituents: HEPES, Potassium chloride, Sucrose, Tergitol-NP40, 100% Loading buffer
  • Concentration information loading...
  • Lysate notesProtein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.
  • Research areas
  • BackgroundEstablished in 1963 from the left maxilla of a 12-year-old African boy with Burkitt's lymphoma, it is the first continuous human hematopoietic cell line. It has become an indispensable tool in haematological research and leukaemia and lymphoma studies.

Applications

Our Abpromise guarantee covers the use of ab30124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

References for Raji whole cell lysate (ab30124)

ab30124 has not yet been referenced specifically in any publications.

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This protein lysate could contain proteases. That’s why we put protease inhibitor cocktail in the protein lyses buffer.


I hope this information is helpful to you. Please do not hesitat...

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Thank you for contacting Abcam Our protein lysate is called total protein lysate, which means most of the proteins, including membrane and surface proteins, are within the lysate. We don’t really have a cell number to generate 100ug lysate, we can only...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"