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Ral Activation Assay Kit (ab212012) utilizes RalBP1 PBD Agarose beads to selectively isolate and pull-down the active form of Ral (RalA and RalB) from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Ral is detected by western blot analysis using an anti-Ral A antibody, which detects the human, mouse, rat, chicken and dog protein.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
The Ral proteins (RalA and RalB) belong to the Ras superfamily. They share 85% identity with each other and 58% with the oncoproteins H-Ras, K-Ras and N-Ras. Both RalA and RalB are 24 kDa proteins that regulate a variety of biological response pathways including vesicle trafficking, oncogenesis, transcription regulation and cell morphology. Like other small GTPases, Ral regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In their active (GTP-bound) state, Ral A and Ral B bind specifically to the protein-binding domain (PBD) of RalBP1 to control downstream signaling cascades.
|Components||20 tests||5 tests|
|ab211181 - RalBP1 PBD Agarose Beads||1 x 800µl||1 x 200µl|
|100X GTPyS||1 x 50µl||1 x 20µl|
|100X GDP||1 x 50µl||1 x 20µl|
|5X Assay/Lysis Buffer||1 x 30ml||4 x 2ml|
|Anti-Ral A Mouse monoclonal antibody||1 x 40µl||1 x 10µl|
|GTPase immunoblot Positive Control||1 x 100µl||1 x 100µl|
Our Abpromise guarantee covers the use of ab212012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab212012 has not yet been referenced specifically in any publications.