Overview

  • Product nameAnti-RALA+RALB antibody
  • Description
    Rabbit polyclonal to RALA+RALB
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Cynomolgus Monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RALA+RALB.

    (Peptide available as ab42531.)

  • Positive control
    • ab42532 gave a positive result in the following Whole Cell Lysates HepG2 MDA MB 361

Properties

Applications

Our Abpromise guarantee covers the use of ab42532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 24 kDa).

Target

Anti-RALA+RALB antibody images

  • All lanes : Anti-RALA+RALB antibody (ab42532) at 1 µg/ml

    Lane 1 : HepG2 whole cell lysate (ab7900)
    Lane 2 : MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)

References for Anti-RALA+RALB antibody (ab42532)

ab42532 has not yet been referenced specifically in any publications.

Product Wall

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