Overview

  • Product name
  • Description
    Mouse polyclonal to RALY
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human RALY (AAI03754.1).

  • Positive control
    • HepG2 cell lysate; RALY transfected 293T cell lysate and HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Probable-RNA binding protein. Could be a heterogeneous nuclear ribonucleoprotein (hnRNP). May be involved in pre-mRNA splicing.
  • Tissue specificity
    Expressed in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Weakly expressed in placenta.
  • Sequence similarities
    Belongs to the RRM HNRPC family. RALY subfamily.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Autoantigen p542 antibody
    • Heterogeneous nuclear ribonucleoprotein C-like 2 antibody
    • hnRNP associated with lethal yellow protein homolog antibody
    • hnRNP core protein C-like 2 antibody
    • MGC117312 antibody
    • P542 antibody
    • RALY antibody
    • RALY_HUMAN antibody
    • RNA binding protein (autoantigenic hnRNP associated with lethal yellow) antibody
    • RNA binding protein (autoantigenic) antibody
    • RNA binding protein autoantigenic (hnRNP associated with lethal yellow homolog (mouse)) antibody
    • RNA-binding protein Raly antibody
    see all

Images

  • Anti-RALY antibody (ab88616) at 1 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • All lanes : Anti-RALY antibody (ab88616) at 1 µg/ml

    Lane 1 : RALY transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • ab88616 at 10µg/ml staining RALY in HeLa cells by Immunofluorescence.

References

ab88616 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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