Ramos whole cell lysate (ab3955)

Overview

  • Product nameRamos whole cell lysate
  • General notesCell line: Ramos (Burkitt's lymphoma).
    Growth media: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, and 10% heat-inactivated FBS.


    Ramos cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • Tested applicationsSuitable for: WBmore details

Properties

  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Lysate notesRamos cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • Research areas
  • BackgroundDerived from a caucasian Burkitt's lymphoma which does not possess the Epstein Barr Virus (EBV) genome. EBV infectability and permanent conversion into EBV positive sub-lines is possible by in vitro infection. The cells have B lymphocyte characteristics, with surface associated mu and kappa chains. Cells are used as model of B lymphocytes and for apoptosis studies.

Applications

Our Abpromise guarantee covers the use of ab3955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. 10 µg to 20 µg per lane is recommended for mini gel.

References for Ramos whole cell lysate (ab3955)

ab3955 has not yet been referenced specifically in any publications.

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The Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab3955) should be stored as directed on the datasheet of this product:
"Upon delivery aliquot and store at -20°C or -80°C."
It is important to...

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Thank you for your inquiry.

I am happy to confirm that Ramos cell do express TLR4 and can be used a positive control for TLR4.

I amnotaware that Ramoscell areunresponsiveto LPS.

Please consider thatI amnot specialised in t...

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Thank you for getting back to me and sorry for the confusion: I have found references to both ramos and RAW cells as positive controls for antibodies targeting TLR7. Therefore I would like to recommend the following lysates: Ramos (Human Burkitt'...

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Thnak you for your enquiry. I have performed a search of the literature and I have found no reference to the use of Ramos cells as a positive control for TLR7. For our own antibodies against TLR7 we make the recommendation of human spleen cells and...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"