Overview

  • Product name
  • Description
    Rabbit polyclonal to RAMP3
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    A synthetic peptide corresponding to N-terminal residues of human RAMP3

Properties

Applications

Our Abpromise guarantee covers the use of ab58660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    RAMP3 (receptor calcitonin activity modifying protein 3) transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. It acts as a receptor for adrenomedullin (AM) together with CALCRL. It is strongly expressed in lung, breast, immune system and fetal tissues and belongs to the RAMP family.
  • Cellular localization
    Cell Membrane; Single-pass type I membrane protein
  • Database links
  • Alternative names
    • Calcitonin receptor like receptor activity modifying protein 3 antibody
    • CRLR activity modifying protein 3 antibody
    • CRLR activity-modifying protein 3 antibody
    • OTTHUMP00000159549 antibody
    • OTTHUMP00000214279 antibody
    • OTTHUMP00000214280 antibody
    • Receptor (calcitonin) activity modifying protein 3 antibody
    • Receptor (G protein-coupled) activity modifying protein 3 antibody
    • Receptor activity modifying protein 3 antibody
    see all

Images

  • ICC/IF image of ab58660 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58660, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Brekhman V  et al. Receptor activity modifying protein-3 mediates the protumorigenic activity of lysyl oxidase-like protein-2. FASEB J : (2010). Read more (PubMed: 20802105) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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