Overview

  • Product nameAnti-Ran antibody
    See all Ran primary antibodies
  • Description
    Rabbit polyclonal to Ran
  • Tested applicationsSuitable for: IHC-P, ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Human, Xenopus laevis
  • Immunogen

    Recombinant full length protein (Human) - His-tagged and purified from E. coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab4781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

  • FunctionGTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
    Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
  • Tissue specificityExpressed in a variety of tissues.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Ran family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationNucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Androgen receptor associated protein 24 antibody
    • Androgen receptor-associated protein 24 antibody
    • ARA 24 antibody
    • ARA24 antibody
    • Gsp1 antibody
    • GTP binding nuclear protein RAN antibody
    • GTP-binding nuclear protein Ran antibody
    • GTPase Ran antibody
    • Guanosine triphosphatase Ran antibody
    • LPS antibody
    • OK/SW-cl.81 antibody
    • ran antibody
    • RAN member RAS oncogene family antibody
    • RAN_HUMAN antibody
    • RanGTPase antibody
    • Ras like protein TC4 antibody
    • Ras related nuclear protein antibody
    • Ras-like protein TC4 antibody
    • Ras-related nuclear protein antibody
    • RASL2 8 antibody
    • TC 4 antibody
    • TC4 antibody
    see all

Anti-Ran antibody images



  • Predicted band size : 26 kDa


    ab4781 at a 1/500 dilution staining approximately 26kDa Ran in Xenopus egg extract (lane X, 50µg per lane) and HeLa total cell extract (lane Hu, 50µg per lane) by western blot (ECL, 1 minute exposure).
  • Anti-Ran antibody (ab4781) at 1/500 dilution + Lysate prepared from human HUH-7 cells at 15000 cells

    Secondary
    HRP conjugated sheep polyclonal to rabbit IgG at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 26 kDa


    Exposure time : 3 minutes

    This image is a courtesy of Anonymous Abreview

    See Abreview

  • IHC image of ab4781 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4781, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Ran antibody (ab4781)

This product has been referenced in:
  • Chen F  et al. A Novel Thyroid Hormone Mediated Regulation of HSV-1 Gene Expression and Replication is Specific to Neuronal Cells and Associated with Disruption of Chromatin Condensation. SOJ Pharm Pharm Sci 1:N/A (2014). Read more (PubMed: 25346944) »
  • Nilsen T  et al. A nuclear export sequence located on a beta-strand in fibroblast growth factor-1. J Biol Chem 282:26245-56 (2007). Read more (PubMed: 17616529) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (huh-7)
Loading amount 15000 cells
Specification huh-7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 25 2009

Thank you for your enquiry. This particular antibody has not been tested in the species that you are looking at, so we can not guarantee that the antibody will work. Since the antibody is working for immunofluorescence, I would think that there could b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"