The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 23308199
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Use at an assay dependent concentration.
FunctionGTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
Tissue specificityExpressed in a variety of tissues.
Sequence similaritiesBelongs to the small GTPase superfamily. Ran family.
Post-translational modificationsThe N-terminus is blocked.
Cellular localizationNucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
All lanes : Anti-Ran antibody (ab53775) at 1/500 dilution
Lane 1 : Extracts from LOVO cells Lane 2 : Extracts from LOVO cells, plus immunising peptide
Predicted band size : 26 kDa Observed band size : 26 kDa
Immunohistochemistry (Paraffin-embedded sections) - Ran antibody (ab53775)
Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using ab53775 at a 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
References for Anti-Ran antibody (ab53775)
This product has been referenced in:
Mastroeni D et al. Reduced RAN expression and disrupted transport between cytoplasm and nucleus; a key event in Alzheimer's disease pathophysiology. PLoS One8:e53349 (2013).
WB, IHC-P, ICC/IF
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