Overview

  • Product nameAnti-Ran antibody
    See all Ran primary antibodies
  • Description
    Rabbit polyclonal to Ran
  • SpecificityThis antibody detects endogenous levels of total Ran protein.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from Human Ran

  • Positive control
    • Human breast carcinoma tissue and extracts from LOVO cells

Properties

Applications

Our Abpromise guarantee covers the use of ab53775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23308199
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • FunctionGTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
    Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
  • Tissue specificityExpressed in a variety of tissues.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Ran family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationNucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Androgen receptor associated protein 24 antibody
    • Androgen receptor-associated protein 24 antibody
    • ARA 24 antibody
    • ARA24 antibody
    • Gsp1 antibody
    • GTP binding nuclear protein RAN antibody
    • GTP-binding nuclear protein Ran antibody
    • GTPase Ran antibody
    • Guanosine triphosphatase Ran antibody
    • LPS antibody
    • OK/SW-cl.81 antibody
    • ran antibody
    • RAN member RAS oncogene family antibody
    • RAN_HUMAN antibody
    • RanGTPase antibody
    • Ras like protein TC4 antibody
    • Ras related nuclear protein antibody
    • Ras-like protein TC4 antibody
    • Ras-related nuclear protein antibody
    • RASL2 8 antibody
    • TC 4 antibody
    • TC4 antibody
    see all

Anti-Ran antibody images

  • All lanes : Anti-Ran antibody (ab53775) at 1/500 dilution

    Lane 1 : Extracts from LOVO cells
    Lane 2 : Extracts from LOVO cells, plus immunising peptide


    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53775 at a 1/50 dilution.

    Left image un-treated.

    Right image treated with immunizing peptide.

References for Anti-Ran antibody (ab53775)

This product has been referenced in:
  • Mastroeni D  et al. Reduced RAN expression and disrupted transport between cytoplasm and nucleus; a key event in Alzheimer's disease pathophysiology. PLoS One 8:e53349 (2013). WB, IHC-P, ICC/IF ; Human . Read more (PubMed: 23308199) »
  • Lecompte S  et al. Genetic and molecular insights into the role of PROX1 in glucose metabolism. Diabetes 62:1738-45 (2013). Read more (PubMed: 23274905) »

See all 4 Publications for this product

Product Wall

Abreviews
Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (U2OS)
Specification U2OS
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jul 09 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"