ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IHC-P.
GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
Expressed in a variety of tissues.
Belongs to the small GTPase superfamily. Ran family.
The N-terminus is blocked.
Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Overlay histogram showing HeLa cells stained with ab155103 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab155103, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot - Anti-Ran antibody [EPR10791(B)] (ab155103)
All lanes : Anti-Ran antibody [EPR10791(B)] (ab155103) at 1/1000 dilution
Lane 1 : HepG2 cell lysate Lane 2 : Lovo cell lysate Lane 3 : Jurkat cell lysate Lane 4 : HeLa cell lysate Lane 5 : C6 cell lysate Lane 6 : RAW264.7 cell lysate Lane 7 : PC12 cell lysate Lane 8 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Zhang H et al. Mechanisms controlling the smooth muscle cell death in progeria via down-regulation of poly(ADP-ribose) polymerase 1. Proc Natl Acad Sci U S A111:E2261-70 (2014).
Read more (PubMed: 24843141) »