Overview

  • Product name
  • Description
    Goat polyclonal to RanBP1
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: LSVKEETKEDAEEKQ, corresponding to amino acids 187-201 of Human RanBP1.

  • Positive control
    • HeLa and HepG2 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab17034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 28-30 kDa (predicted molecular weight: 23 kDa).Can be blocked with Human RanBP1 peptide (ab23305).
ELISA 1/16000.

Target

  • Function
    Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.
  • Sequence similarities
    Belongs to the RANBP1 family.
    Contains 1 RanBD1 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • HTF9A antibody
    • RAN binding protein 1 antibody
    • Ran specific GTPase activating protein antibody
    • Ran-binding protein 1 antibody
    • Ran-specific GTPase-activating protein antibody
    • RANBP1 antibody
    • RANG_HUMAN antibody
    see all

Images

  • ab17034 (3.8µg/ml) staining of paraffin embedded Human Kidney tissue following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows pixulated cytoplasm staining in DCT.

  • Anti-RanBP1 antibody (ab17034) at 0.05 µg/ml + HeLa cell Lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 23 kDa

    Primary Incubation 1 hour.



  • Predicted band size : 23 kDa
    ab17034 at 0.1µg/ml staining RanBP1 from HepG2 lysate (35µg total protein per lane) by Western blot. The primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence. ab17034 at 0.1µg/ml staining RanBP1 from HepG2 lysate (35µg total protein per lane) by Western blot. The primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence.

References

ab17034 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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