The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 28-30 kDa (predicted molecular weight: 23 kDa).Can be blocked with Human RanBP1 peptide (ab23305).
FunctionInhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.
Sequence similaritiesBelongs to the RANBP1 family. Contains 1 RanBD1 domain.
ab17034 (3.8µg/ml) staining of paraffin embedded Human Kidney tissue following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows pixulated cytoplasm staining in DCT.
Western blot - Anti-RanBP1 antibody (ab17034)
Anti-RanBP1 antibody (ab17034) at 0.05 µg/ml + HeLa cell Lysate in RIPA buffer at 35 µg developed using the ECL technique
Predicted band size : 23 kDa
Primary Incubation 1 hour.
Western blot - RanBP1 antibody (ab17034)
Predicted band size : 23 kDa ab17034 at 0.1µg/ml staining RanBP1 from HepG2 lysate (35µg total protein per lane) by Western blot. The primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence. ab17034 at 0.1µg/ml staining RanBP1 from HepG2 lysate (35µg total protein per lane) by Western blot. The primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence.
References for Anti-RanBP1 antibody (ab17034)
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