Overview

  • Product name
  • Description
    Rabbit polyclonal to RanBP1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding a region between residues 1 and 50 of human RanBP1 (NP_002873.1)

  • Positive control
    • HeLa whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/1000 - 1/5000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.
    • Sequence similarities
      Belongs to the RANBP1 family.
      Contains 1 RanBD1 domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • HTF9A antibody
      • RAN binding protein 1 antibody
      • Ran specific GTPase activating protein antibody
      • Ran-binding protein 1 antibody
      • Ran-specific GTPase-activating protein antibody
      • RANBP1 antibody
      • RANG_HUMAN antibody
      see all

    Images

    • Lane 1 : Anti-RanBP1 antibody (ab70185) at 1 µg/ml
      Lane 2 : Anti-RanBP1 antibody (ab70185) at 0.33 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : HeLa whole cell lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 23 kDa
      Observed band size : 28 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes
    • Western Blot showing ab70185 at 1µg/ml staining RanBP1 in HeLa whole cell lysates immunoprecipitated using ab70185 at 5µg/0.5mg lysate (Lane 1). Lane 2: Control IgG was used for immunoprecipitation. Detection: Chemiluminescence with an exposure time of 5 minutes

    References

    ab70185 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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