The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 124 kDa).Can be blocked with Human RanBP16 peptide (ab23146).
RelevanceThe transport of protein and large RNAs through the nuclear pore complexes (NPC) is an energy-dependent and regulated process. The import of proteins with a nuclear localization signal (NLS) is accomplished by recognition of one or more clusters of basic amino acids by the importin-alpha/beta complex. The small GTPase RAN plays a key role in NLS-dependent protein import. RAN-binding protein-16 is a member of the importin-beta superfamily of nuclear transport receptors.
Cellular localizationCytoplasm. Nucleus. Nucleus; nuclear pore complex. Note: Shuttles between the nucleus and the cytoplasm