Overview

  • Product name
  • Description
    Rabbit polyclonal to RanBP2
  • Specificity
    Detects Ran binding protein 2 (RanBP 2) from human and bovine retina.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human RanBP2 aa 1-18.
    Sequence:

    MRRSKADVERYIASVQGS


    (Peptide available as ab4939)

  • Positive control
    • Bovine retinal extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab2938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.

Immunohistochemical staining of bovine retina results in cytoplasmic staining of cells within the inner nuclear layer as well as the ganglion cells.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 360 kDa (predicted molecular weight: 358 kDa).Can be blocked with RanBP2 peptide (ab4939).

Detects a band of approximately 360 kDa representing RanBP 2 from bovine retinal extracts.

Target

  • Function
    E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1.
  • Pathway
    Protein modification; protein sumoylation.
  • Involvement in disease
    Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem.
  • Sequence similarities
    Contains 1 PPIase cyclophilin-type domain.
    Contains 4 RanBD1 domains.
    Contains 8 RanBP2-type zinc fingers.
    Contains 1 TPR repeat.
  • Domain
    Contains F-X-F-G repeats.
  • Post-translational
    modifications
    Polyubiquitinated by PARK2, which leads to proteasomal degradation.
  • Cellular localization
    Nucleus > nuclear pore complex. Cytoplasmic filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • 358 kDa nucleoporin antibody
    • ANE1 antibody
    • E3 SUMO-protein ligase RanBP2 antibody
    • IIAE3 antibody
    • Nuclear pore complex protein Nup358 antibody
    • Nucleoporin 358 antibody
    • Nucleoporin Nup358 antibody
    • NUP358 antibody
    • p270 antibody
    • RAN binding protein 2 antibody
    • Ran-binding protein 2 antibody
    • RANBP2 antibody
    • RBP2_HUMAN antibody
    • Transformation related protein 2 antibody
    • TRP 1 antibody
    • TRP 2 antibody
    • TRP1 antibody
    • TRP2 antibody
    see all

Images

  • Immunohistochemical staining of RanBP2 in bovine retina using ab2938.



  • Predicted band size : 358 kDa
    Western blot of RanBP2 from human retinal extracts using ab2938. Western blot of RanBP2 from human retinal extracts using ab2938.

References

This product has been referenced in:
  • Saito A  et al. Roles of Capsid-Interacting Host Factors in Multimodal Inhibition of HIV-1 by PF74. J Virol 90:5808-23 (2016). Read more (PubMed: 27076642) »
  • Henning MS  et al. In vivo functions of CPSF6 for HIV-1 as revealed by HIV-1 capsid evolution in HLA-B27-positive subjects. PLoS Pathog 10:e1003868 (2014). WB ; Human . Read more (PubMed: 24415937) »

See all 8 Publications for this product

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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