Overview

  • Product name
  • Description
    Rabbit polyclonal to RanBP3
  • Specificity
    Detects Ran binding protein 3 (RanBP 3) from human and rat cells.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human RanBP3 aa 1-13.
    Sequence:

    MADLANEEKPAIA


    (Peptide available as ab4940)

  • Positive control
    • HeLa cell lysate. NRK cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab2939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.

Immunofluorescence staining of RanBP 3 in NRK cells results in diffuse nuclear staining.

WB Use a concentration of 10 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).Can be blocked with RanBP3 peptide (ab4940).

Target

  • Function
    Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export.
  • Tissue specificity
    Widely expressed with high levels in testis and heart.
  • Sequence similarities
    Contains 1 RanBD1 domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp586I1520 antibody
    • Ran binding protein 3 antibody
    • Ran-binding protein 3 antibody
    • RANB3_HUMAN antibody
    • RanBP3 antibody
    see all

Images

  • Immunohistochemical staining of RanBP3 in NRK cells using ab2939.


  • Predicted band size : 60 kDa


    Western blot on RanBP3 in HeLa cytosol using ab2939

References

This product has been referenced in:
  • Khalil H  et al. The caspase-3-p120-RasGAP module generates a NF-?B repressor in response to cellular stress. J Cell Sci 128:3502-13 (2015). Read more (PubMed: 26224876) »
  • Zhang R  et al. Perturbation of host nuclear membrane component RanBP2 impairs the nuclear import of human immunodeficiency virus -1 preintegration complex (DNA). PLoS One 5:e15620 (2010). WB ; Human . Read more (PubMed: 21179483) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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