The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. The use of citrate buffer will enhance staining. Likely to work in frozen sections.
FunctionActs as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export.
Tissue specificityWidely expressed with high levels in testis and heart.
Sequence similaritiesContains 1 RanBD1 domain.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
ab84672 at 1/100 dilution staining RanBP3 in Human colon carcinoma by Immunofluorescence Formalin-fixed, Paraffin-embedded tissue. Detection: Red-fluorescent goat antirabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 at a dilution of 1/100.