Overview

  • Product name
    Anti-RanBP3 antibody [AT12E11]
    See all RanBP3 primary antibodies
  • Description
    Mouse monoclonal [AT12E11] to RanBP3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus monkey, Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human RanBP3 aa 235-445. Purified from E. coli. NCBI Accession No.: NP_015559
    Sequence:

    ISSSLE NSTNSADASS NKFVFGQNMS ERVLSPPKLN EVSSDANREN AAAESGSESS SQEATPEKESLAESAAAYTK ATARKCLLEK VEVITGEEAE SNVLQMQCKL FVFDKTSQSW VERGRGLLRLNDMASTDDGT LQSRLVMRTQ GSLRLILNTK LWAQMQIDKA SEKSIRITAM DTEDQGVKVFLISASSKDTG QLYAALHHRI LALRS


    Database link: Q9H6Z4-3

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab194212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 53 kDa.

Target

  • Function
    Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export.
  • Tissue specificity
    Widely expressed with high levels in testis and heart.
  • Sequence similarities
    Contains 1 RanBD1 domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp586I1520 antibody
    • Ran binding protein 3 antibody
    • Ran-binding protein 3 antibody
    • RANB3_HUMAN antibody
    • RanBP3 antibody
    see all

Images

  • Anti-RanBP3 antibody [AT12E11] (ab194212) at 1/500 dilution + Jurkat cell lysate at 40 µg

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 53 kDa

    PVDF membrane.

References

ab194212 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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