• Product nameAnti-RANBP6 antibody
    See all RANBP6 primary antibodies
  • Description
    Rabbit polyclonal to RANBP6
  • Specificityab74448 detects endogenous levels of total RANBP6 protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human RANBP6.

  • Positive control
    • K562, COLO and COS7 cell extracts. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney. IF/ICC: A549 cell line.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab74448 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab74448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 125 kDa.
ELISA 1/10000.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.


  • RelevanceRANBP6 (Ran-binding protein 6) belongs to the importin beta family and may function in nuclear protein import as a nuclear transport receptor.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • C630001B19 antibody
    • FLJ11120 antibody
    • FLJ54311 antibody
    • OTTHUMP00000021039 antibody
    • OTTHUMP00000021040 antibody
    • RAN binding protein 6 antibody
    • Ran GTP binding protein antibody
    • RANBP 6 antibody
    see all

Anti-RANBP6 antibody images

  • ICC/IF image of ab74448 stained A549 cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74448, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • All lanes : Anti-RANBP6 antibody (ab74448) at 1/500 dilution

    Lane 1 : K562 cell extracts
    Lane 2 : COLO cell extracts
    Lane 3 : COS7 cell extracts
    Lane 4 : COS7 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 125 kDa
    Observed band size : 125 kDa
  • IHC image of RANBP6 staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74448, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

References for Anti-RANBP6 antibody (ab74448)

ab74448 has not yet been referenced specifically in any publications.

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