Overview

  • Product name
  • Description
    Goat polyclonal to RanBP9
  • Tested applications
    Suitable for: IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Dog, Pig
  • Immunogen

    Synthetic peptide:

    GSCAFATVEDYLH

    , corresponding to C terminal amino acids 717-729 of Human RanBP9.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 21561988
ELISA Use at an assay dependent dilution. Peptide ELISA: Antibody detection limit dilution 1:16,000
IHC-P Use at an assay dependent concentration.

Target

  • Function
    May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Involved in activation of androgen and glucocorticoid receptor in the presence of their cognate hormones. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA.
  • Tissue specificity
    Ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung. Within the brain, expressed predominantly by neurons in the gray matter of cortex, the granular layer of cerebellum and the Purkinje cells.
  • Sequence similarities
    Belongs to the RANBP9/10 family.
    Contains 1 B30.2/SPRY domain.
    Contains 1 CTLH domain.
    Contains 1 LisH domain.
  • Domain
    The SPRY domain mediates the interaction with MET, AR, and CDC2L1.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated in response to stress. Can be phosphorylated by the cleaved p110 form of CDC2L1 (p110C).
    Ubiquitinated. Polyubiquitination targets the protein for rapid degradation via the ubiquitin system. Can be deubiquitinated by USP11.
  • Cellular localization
    Nucleus. Cytoplasm. A phosphorylated form is associated with the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell antigen receptor Ig beta associated protein 1 antibody
    • BPM 90 antibody
    • BPM L antibody
    • BPM-L antibody
    • BPM90 antibody
    • BPML antibody
    • IBAP 1 antibody
    • Imp 9 antibody
    • Importin 9 antibody
    • Novel centrosomal protein RanBPM antibody
    • RAN binding protein 9 antibody
    • Ran binding protein centrosomal antibody
    • Ran Binding Protein in the Microtubule organizing center antibody
    • Ran binding protein M antibody
    • Ran BP9 antibody
    • Ran-binding protein 9 antibody
    • Ran-binding protein M antibody
    • RANB9_HUMAN antibody
    • RanBP 7 antibody
    • RANBP 9 antibody
    • RanBP7 antibody
    • RanBP9 antibody
    • RanBPM antibody
    see all

Anti-RanBP9 antibody images

  • Ab5295 (3ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

References for Anti-RanBP9 antibody (ab5295)

This product has been referenced in:
  • Salemi LM  et al. Characterization of RanBPM molecular determinants that control its subcellular localization. PLoS One 10:e0117655 (2015). ICC/IF . Read more (PubMed: 25659156) »
  • Tsioras K  et al. Functional Interactions between BM88/Cend1, Ran-Binding Protein M and Dyrk1B Kinase Affect Cyclin D1 Levels and Cell Cycle Progression/Exit in Mouse Neuroblastoma Cells. PLoS One 8:e82172 (2013). Mouse . Read more (PubMed: 24312406) »

See all 5 Publications for this product

Product Wall

Thank you for your enquiry. This antibody is a Fast-Track antibody. It has not been fully characterized in Western blot. As such, we cannot guarantee its use in Western blotting; however, you may be able to earn 1000 Abpoints for being the first s...

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Thank you for your enquiry. The synthetic peptide used to raise ab5295, anti-RanBP9 was GSCAFATVEDYLH, corresponding to C terminal amino acids 717-729 of Human RanBP9. Unfortunately the epitope of the antiserum has not been mapped. I hope this i...

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Thank you very much for contacting us regarding ab5295. I am very sorry to hear that you are experiencing difficulty with this antibody. As you may know, ab5295 is one of our Fast-track antibodies and it is not yet fully characterized. We cannot gua...

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Thank you for your enquiry. ab5295 is currently a fast-track antibody and has not been fully characterized. According to the datasheet there is a 76% identity between the immunogen and the corresponding rat homolog. I would therefore consider it hi...

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Thank you for your enquiry. I'm afraid that I can't send our customer's blot to you, all of our customer information is confidential. As stated on the datasheet, ab5295 is a Fast-Track antibody; Fast-Track antibodies are immunogen affinity purified and...

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Thank you for getting back to us and for sharing your results with us. As you probably know, this is a Fast-track antibody and it has not been fully characterized. We have read through your protocol and would like to make the following suggestio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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